Co-production of hydrogen and ethanol from glucose in by activation of pentose-phosphate pathway through deletion of phosphoglucose isomerase () and overexpression of glucose-6-phosphate dehydrogenase () and 6-phosphogluconate dehydrogenase ().

Citation data:

Biotechnology for biofuels, ISSN: 1754-6834, Vol: 10, Issue: 1, Page: 85

Publication Year:
2017
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Repository URL:
http://scholarworks.unist.ac.kr/handle/201301/22228
PMID:
28360941
DOI:
10.1186/s13068-017-0768-2
Author(s):
Sundara Sekar, Balaji; Seol, Eunhee; Park, Sunghoon
Publisher(s):
Springer Nature; BIOMED CENTRAL LTD
Tags:
Biochemistry, Genetics and Molecular Biology; Immunology and Microbiology; Energy; Environmental Science; Biohydrogen; Co-production of hydrogen and ethanol; Phosphoglucose isomerase deletion; Pentose-phosphate pathway; Escherichia coli
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article description
Biologically, hydrogen (H) can be produced through dark fermentation and photofermentation. Dark fermentation is fast in rate and simple in reactor design, but H production yield is unsatisfactorily low as <4 mol H/mol glucose. To address this challenge, simultaneous production of H and ethanol has been suggested. Co-production of ethanol and H requires enhanced formation of NAD(P)H during catabolism of glucose, which can be accomplished by diversion of glycolytic flux from the Embden-Meyerhof-Parnas (EMP) pathway to the pentose-phosphate (PP) pathway in . However, the disruption of (hospholucose somerase) for complete diversion of carbon flux to the PP pathway made unable to grow on glucose under anaerobic condition.