A parameter estimation method for fluorescence lifetime data.

Citation data:

BMC research notes, ISSN: 1756-0500, Vol: 8, Issue: 1, Page: 230

Publication Year:
2015
Usage 33
Full Text Views 16
Abstract Views 12
Link-outs 5
Repository URL:
http://scholarworks.unist.ac.kr/handle/201301/16798
PMID:
26054354
DOI:
10.1186/s13104-015-1176-y
PMCID:
PMC4467687
Author(s):
Sewell, Daniel; Kim, Hajin; Ha, Taek Jip; Ma, Ping
Publisher(s):
Springer Nature; BioMed Central
Tags:
Biochemistry, Genetics and Molecular Biology; Mixture distribution; Numerical optimization; Overfitting data; Single-molecule fluorescence lifetime
article description
When modeling single-molecule fluorescence lifetime experimental data, the analysis often involves fitting a biexponential distribution to binned data. When dealing with small sample sizes, there is the potential for convergence failure in numerical optimization, for convergence to local optima resulting in physically unreasonable parameter estimates, and also for overfitting the data.