Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood.

Citation data:

Journal of visualized experiments : JoVE, ISSN: 1940-087X, Vol: 2016, Issue: 110, Page: 54143

Publication Year:
2016
Usage 25
Clicks 15
Abstract Views 7
Link-outs 3
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Repository URL:
http://scholarworks.unist.ac.kr/handle/201301/19189
PMID:
27167836
DOI:
10.3791/54143
PMCID:
PMC4941934
Author(s):
Park, Yang-Seok; Sunkara, Vijaya; Kim, Yubin; Lee, Won Seok; Han, Ja-Ryoung; Cho, Yoon-Kyoung
Publisher(s):
MyJove Corporation; MYJoVE Corporation
Tags:
Neuroscience; Chemical Engineering; Biochemistry, Genetics and Molecular Biology; Immunology and Microbiology; Bioengineering; Electrospinning; Full automation; Immunoassay; Issue 110; Lab-on-a-disc; Low-volume assay; On-disc detection; Tio2 nanofibers; Transfer-printing; Ultrasensitive; Whole blood
article description
Enzyme-linked immunosorbent assay (ELISA) is a promising method to detect small amount of proteins in biological samples. The devices providing a platform for reduced sample volume and assay time as well as full automation are required for potential use in point-of-care-diagnostics. Recently, we have demonstrated ultrasensitive detection of serum proteins, C-reactive protein (CRP) and cardiac troponin I (cTnI), utilizing a lab-on-a-disc composed of TiO2 nanofibrous (NF) mats. It showed a large dynamic range with femto molar (fM) detection sensitivity, from a small volume of whole blood in 30 min. The device consists of several components for blood separation, metering, mixing, and washing that are automated for improved sensitivity from low sample volumes. Here, in the video demonstration, we show the experimental protocols and know-how for the fabrication of NFs as well as the disc, their integration and the operation in the following order: processes for preparing TiO2 NF mat; transfer-printing of TiO2 NF mat onto the disc; surface modification for immune-reactions, disc assembly and operation; on-disc detection and representative results for immunoassay. Use of this device enables multiplexed analysis with minimal consumption of samples and reagents. Given the advantages, the device should find use in a wide variety of applications, and prove beneficial in facilitating the analysis of low abundant proteins.