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Antibodies against GM ganglioside affect K and NA currents in isolated rat myelinated nerve fibers

Annals of Neurology, ISSN: 1531-8249, Vol: 37, Issue: 4, Page: 436-442
1995
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Article Description

High titers of anti‐GM ganglioside antibodies (anti‐GM antibodies) may be implicated in lower motor neuron disease. We studied the pathogenic role of anti‐GM antibody using the petroleum jelly–gap voltage clamp technique on isolated single myelinated rat nerve fibers. Anti‐GM antisera were obtained from rabbits immunized with GM ganglioside. Extracellularly applied anti‐GM antisera without complement activity increased both the rate of rise and the amplitude of the K current elicited by step depolarization, with little effect on Na current. In the presence of active complement, however, anti‐GM antibodies decreased the Na current, and caused a progressive increase of nonspecific leakage current. Neither complement alone nor complement‐supplemented antisera from which anti‐GM antibodies were depleted by affinity chromatography had any effect on ionic current. These observations indicate that anti‐GM antibodies themselves can uncover K channels in the paranodal region, while anti‐GM antibodies bound to the nodal membrane in the presence of complement may form antibody‐complement complexes that block Na channels and disrupt the membrane at the node of Ranvier. Copyright © 1995 American Neurological Association

Bibliographic Details

Tomoko Takigawa; Hitoshi Yasuda; Ryuichi Kikkawa; Yukio Shigeta; Hiroshi Kitasato; Takahiko Saida

Wiley

Neuroscience; Medicine

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