PlumX Metrics
Embed PlumX Metrics

Targeted gene delivery to CD117-expressing cells in vivo with lentiviral vectors co-displaying stem cell factor and a fusogenic molecule

Biotechnology and Bioengineering, ISSN: 0006-3592, Vol: 104, Issue: 1, Page: 206-215
2009
  • 17
    Citations
  • 0
    Usage
  • 35
    Captures
  • 1
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    17
  • Captures
    35
  • Mentions
    1
    • News Mentions
      1
      • News
        1

Most Recent News

A road to survival

Te'Avionna Rowe, or Te’A as most people call her, is a bright 14-year-old and captain of her junior varsity cheerleading squad in Florida. She also

Article Description

The development of a lentiviral system to deliver genes to specific cell types could improve the safety and the efficacy of gene delivery. Previously, we have developed an efficient method to target lentivectors to specific cells via an antibody-antigen interaction in vitro and in vivo. We report herein a targeted lentivector that harnesses the natural ligand-receptor recognition mechanism for targeted modification of c-KIT receptor-expressing cells. For targeting, we incorporate membrane-bound human stem cell factor (hSCF), and for fusion, a Sindbis virus-derived fusogenic molecule (FM) onto the lentiviral surface. These engineered vectors can recognize cells expressing surface CD117, resulting in efficient targeted transduction of cells in an SCF-receptor dependent manner in vitro, and in vivo in xenografted mouse models. This study expands the ability of targeting lentivectors beyond antibody targets to include cell-specific surface receptors. Development of a high titer lentivector to receptor-specific cells is an attractive approach to restrict gene expression and could potentially ensure therapeutic effects in the desired cells while limiting side effects caused by gene expression in non-target cells. © 2009 Wiley Periodicals, Inc.

Bibliographic Details

Froelich, Steven; Ziegler, Leslie; Stroup, Katie; Wang, Pin

Wiley

Biochemistry, Genetics and Molecular Biology; Chemical Engineering; Immunology and Microbiology

Provide Feedback

Have ideas for a new metric? Would you like to see something else here?Let us know