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Binding of mammalian brain microtubule-associated proteins (MAPs) to insect ovarian microtubules.

Cell motility and the cytoskeleton, ISSN: 0886-1544, Vol: 8, Issue: 2, Page: 174-181
1987
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In this study we have applied microtubule-associated proteins (MAPs) from mammalian brain to both native and reassembled insect ovarian microtubules. Such microtubules, which are normally smooth walled, become decorated with projections similar to those observed when mammalian brain MAPs are added back to assembling or assembled mammalian brain microtubules. The mammalian MAPs were also detected as components of insect microtubules when analyzed by polyacrylamide gel electrophoresis. Our observations suggest that mammalian brain MAPs have common binding sites on microtubules from two widely different sources and indicate the degree of evolutionary conservation of such sites.

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