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Identification of a NCR/NKG2D/LFA-1 /CD94 immature human NK cell subset

Cytometry Part A, ISSN: 1552-4922, Vol: 75, Issue: 11, Page: 893-901
2009
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Article Description

CD56 natural killer (NK) cells, generated in vitro from CD34 hematopoietic progenitor cells, were characterized after a 30-day culture with flt3 ligand plus IL-15. Virtually, all CD56 cells expressed CD117, CD25, natural cytotoxicity receptors (NCRs), NKG2D, CD161, and CD244, while only a subset expressed CD18-CD11a (LFA-1), and CD94 molecule, defining an immature CD56/NCRs/NKG2D / LFA-1/CD94 subset. Another small subset of cells expressing CD94 but not LFA-1 integrin was also identified, suggesting that during NK differentiation LFA-1 might be upregulated later than CD94. To verify this hypothesis in vivo, we evaluated the NK cell expression of LFA-1 in both peripheral and umbilical cord blood samples. Interestingly, in these blood fluids, we have identified a lineage negative CD34/LFA-1 /NKp46/ NKG2D/CD94 subset that resembled an immature stage of NK cells present in lymph nodes. Altogether, the results indicate that CD18-CD11a integrin, as well as CD11b in mice, may be a useful marker to identify immature stages of NK cell differentiation. © 2009 International Society for Advancement of Cytometry.

Bibliographic Details

Zamai, Loris; Galeotti, Laura; Del Zotto, Genny; Canonico, Barbara; Mirandola, Prisco; Papa, Stefano

Wiley

Medicine; Biochemistry, Genetics and Molecular Biology

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