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Identification of DOCK4 and its splicing variant as PIP binding proteins

IUBMB Life, ISSN: 1521-6543, Vol: 60, Issue: 7, Page: 467-472
2008
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Article Description

DOCK4, a member of DOCK180 family proteins, was originally identified as a product of a gene deleted during tumor progression. Although its tumor suppression properties have been reported, the regulation mechanism of this protein has not been fully elucidated. DOCK4 shares two conserved domains called as DHR-1 and DHR-2 domain as other members including DOCK180. Although DHR-1 in DOCK180 is reported to bind to PIP, whether that of DOCK4 exhibits similar function has yet not been examined. In a search for novel PIP binding proteins by the PIP analog beads binding assay, we found that DOCK4 and its novel splicing variant, whose exon1 and exon52 are different from the known one, bind to PIP. Binding assay using deletion mutants of DOCK4 revealed that the binding region falls into the DHR-1 domain. These results raise the possibility that DOCK4 may be regulated by PIP to exert its function. © 2008 IUBMB.

Bibliographic Details

Kanai, Akinori; Ihara, Sayoko; Ohdaira, Tsutomu; Shinohara-Kanda, Azusa; Iwamatsu, Akihiro; Fukui, Yasuhisa

Wiley

Biochemistry, Genetics and Molecular Biology

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