PlumX Metrics
Embed PlumX Metrics

A cotton-specific gene, stearoyl-ACP desaturase, used as a reference for qualitative and real-time quantitative polymerase chain reaction detection of genetically modified organisms

Journal of the Science of Food and Agriculture, ISSN: 0022-5142, Vol: 86, Issue: 7, Page: 1103-1109
2006
  • 18
    Citations
  • 0
    Usage
  • 11
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    18
    • Citation Indexes
      18
  • Captures
    11

Article Description

Biotechnology has permitted the modification of agricultural materials in a very precise way to improve productivity and yields. Polymerase chain reaction (PCR)-based methods have been the first choice of most analytical laboratories for routine use in the detection of genetically modified organisms (GMO) and their derived products. These methods rely on the amplification of transgenic sequences and quantification of the transgenic DNA by comparison with an amplified reference gene. This paper describes the specific primers and probe for the cotton stearoyl-ACP desaturase (sad1) gene, and PCR cycling conditions suitable for the use of this sequence, which acts as an endogenous reference gene in both qualitative and quantitative PCR assays. The two methods were tested with 18 cotton varieties and identical amplification products were obtained with all of them. No amplification products were detected when DNA samples from other species, including soybean, rapeseed, tobacco, maize, tomato, potato, cucumber, pea, red pepper, sunflower, sesame, rice, peach, banana, apple, pumpkin, barley and carrot, were used as templates, which demonstrates that this system is specific for cotton. In real-time quantitative PCR analysis, the detection limit was as low as 6 pg of DNA, which indicates that this method is suitable for application to processed food samples that contain very low copies of target DNA. Southern blot analysis confirmed that the sad1 gene was a single copy in the tested cotton varieties. © 2006 Society of Chemical Industry.

Bibliographic Details

Wen Tao Xu; Kun Lun Huang; Ying Wang; Hong Xing Zhang; Yun Bo Luo

Wiley

Biochemistry, Genetics and Molecular Biology; Agricultural and Biological Sciences; Nursing

Provide Feedback

Have ideas for a new metric? Would you like to see something else here?Let us know