A family of low and high copy replicative, integrative and single‐stranded S. cerevisiae/E. coli shuttle vectors
Yeast, ISSN: 1097-0061, Vol: 7, Issue: 6, Page: 609-615
1991
- 528Citations
- 83Captures
- 1Mentions
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations528
- Citation Indexes527
- 527
- CrossRef401
- Patent Family Citations1
- Patent Families1
- Captures83
- Readers83
- 83
- Mentions1
- Blog Mentions1
- Blog1
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Article Description
We describe a set of replicative, integrative and single‐stranded shuttle vectors constructed from the pUC19 plasmid that we use routinely in our experiments. They bear a yeast selectable marker: URA3, TRP1 or LEU2. Replicative vectors carrying different yeast replication origins have been constructed in order to have plasmids based on the same construction with a high or low copy number per cell and with different mitotic stabilities. All the vectors are small in size, provide a high yield in Escherichia coli and efficiently transform Saccharomyces cerevisiae. These plasmids have many of the unique sites of the pUC19 multicloning region and many of them allow for the screening of plasmids with an insert by alpha‐complementation. The nucleotide sequence of each of them is completely known. Copyright © 1991 John Wiley & Sons Ltd.
Bibliographic Details
Wiley
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