Measuring the Replicative Lifespan of Saccharomyces cerevisiae Using the HYAA Microfluidic Platform
Methods in Molecular Biology, ISSN: 1940-6029, Vol: 2144, Page: 1-6
2020
- 5Citations
- 11Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations5
- Citation Indexes5
- Captures11
- Readers11
- 11
Book Chapter Description
The replicative aging of the budding yeast, Saccharomyces cerevisiae, has been a useful model for dissecting the molecular mechanisms of the aging process. Traditionally, the replicative lifespan (RLS) is measured by manually dissecting mother cells from daughter cells, which is a very tedious process. Since 2012, several microfluidic systems have been developed to automate the dissection process, significantly accelerating RLS determination. Here, we describe a detailed protocol of RLS measurement using a ommercially available microfluidic system based on the HYAA chip design, which enables data collection of up to 8000 cells in a single experiment.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85085155153&origin=inward; http://dx.doi.org/10.1007/978-1-0716-0592-9_1; http://www.ncbi.nlm.nih.gov/pubmed/32410020; http://link.springer.com/10.1007/978-1-0716-0592-9_1; https://dx.doi.org/10.1007/978-1-0716-0592-9_1; https://link.springer.com/protocol/10.1007/978-1-0716-0592-9_1
Springer Science and Business Media LLC
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