Imaging Plant Cells by High-Pressure Freezing and Serial block-face scanning electron microscopy
Methods in Molecular Biology, ISSN: 1940-6029, Vol: 2177, Page: 69-81
2020
- 13Citations
- 8Captures
Metric Options: CountsSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations13
- Citation Indexes13
- 13
- Captures8
- Readers8
Book Chapter Description
This chapter describes methods to enhanced contrast of plant material processed by high-pressure freezing and freeze substitution for improved visualization by serial block-face scanning electron microscopy (SBEM). The contrast enhancing steps are based on a protocol involving the sequential incubation of samples in heavy metals and sodium thiocarbohydrazide (OTO staining). We also describe the pipeline for imaging plant tissues in a commercial SBEM system (Gatan 3View) and routines for the image analysis and three-dimensional reconstructions using open-source and commercial software packages.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85087632439&origin=inward; http://dx.doi.org/10.1007/978-1-0716-0767-1_7; http://www.ncbi.nlm.nih.gov/pubmed/32632806; http://link.springer.com/10.1007/978-1-0716-0767-1_7; https://dx.doi.org/10.1007/978-1-0716-0767-1_7; https://link.springer.com/protocol/10.1007/978-1-0716-0767-1_7
Springer Science and Business Media LLC
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