Detection of 5-formylcytosine in Mitochondrial Transcriptome
Methods in Molecular Biology, ISSN: 1940-6029, Vol: 2192, Page: 59-68
2021
- 6Citations
- 2Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations6
- Citation Indexes6
- Captures2
- Readers2
Book Chapter Description
Posttranscriptional RNA modifications have recently emerged as essential posttranscriptional regulators of gene expression. Here we present two methods for single nucleotide resolution detection of 5-formylcytosine (fC) in RNA. The first relies on chemical protection of fC against bisulfite treatment, the second method is based on chemical reduction of fC to hmC. In combination with regular bisulfite treatment of RNA, the methods allow for precise mapping of fC. The protocol is used for fC detection in mtDNA-encoded RNA, however, it can be straightforwardly applied for transcriptome-wide analyses.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85096728020&origin=inward; http://dx.doi.org/10.1007/978-1-0716-0834-0_5; http://www.ncbi.nlm.nih.gov/pubmed/33230765; http://link.springer.com/10.1007/978-1-0716-0834-0_5; https://dx.doi.org/10.1007/978-1-0716-0834-0_5; https://link.springer.com/protocol/10.1007/978-1-0716-0834-0_5
Springer Science and Business Media LLC
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