Shed-Microspore Culture in Ornamental Peppers for Doubled Haploid Plant Production
Methods in Molecular Biology, ISSN: 1940-6029, Vol: 2288, Page: 251-266
2021
- 1Citations
- 8Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations1
- Citation Indexes1
- Captures8
- Readers8
Book Chapter Description
The shed-microspore culture technique is an alternative sub-method combining anther and isolated microspore culture to induce microspore embryogenesis. Recently, its effective use in different types of peppers has drawn attention, because it has a higher embryo yield potential compared to anther culture and is more practical than isolated microspore culture. In this chapter, a stepwise protocol for shed-microspore culture of ornamental pepper is described. This protocol includes the steps of donor plant growth conditions, the choice of suitable flower buds based on DAPI staining of microspores, application of a cold pretreatment to flower buds, surface sterilization of the buds, shed-microspore culture of anthers, stress treatments, regeneration of androgenic in vitro plantlets, their acclimatization and ploidy analysis, and in vivo chromosome doubling of the haploid plants.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85111770658&origin=inward; http://dx.doi.org/10.1007/978-1-0716-1335-1_15; http://www.ncbi.nlm.nih.gov/pubmed/34270016; https://link.springer.com/10.1007/978-1-0716-1335-1_15; https://dx.doi.org/10.1007/978-1-0716-1335-1_15; https://link.springer.com/protocol/10.1007/978-1-0716-1335-1_15
Springer Science and Business Media LLC
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