Quantitative analysis of myofibroblast contraction by traction force microscopy
Methods in Molecular Biology, ISSN: 1940-6029, Vol: 2299, Page: 181-195
2021
- 1Citations
- 19Captures
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Book Chapter Description
Myofibroblasts play important roles in physiological processes such as wound healing and tissue repair. While high contractile forces generated by the actomyosin network enable myofibroblasts to physically contract the wound and bring together injured tissue, prolonged and elevated levels of contraction also drive the progression of fibrosis and cancer. However, quantitative mapping of these forces has been difficult due to their extremely low magnitude ranging from 100 pN/μm to 2 nN/μm. Here, we provide a protocol to measure cellular forces exerted on two-dimensional compliant elastic hydrogels. We describe the fabrication of polyacrylamide hydrogels labeled with fluorescent fiducial markers, functionalization of substrates with ECM proteins, setting up the experiment, and imaging procedures. We demonstrate the application of this technique for quantitative analysis of traction forces exerted by myofibroblasts.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85107040287&origin=inward; http://dx.doi.org/10.1007/978-1-0716-1382-5_14; http://www.ncbi.nlm.nih.gov/pubmed/34028744; https://link.springer.com/10.1007/978-1-0716-1382-5_14; https://dx.doi.org/10.1007/978-1-0716-1382-5_14; https://link.springer.com/protocol/10.1007/978-1-0716-1382-5_14
Springer Science and Business Media LLC
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