CRISPR Interference (CRISPRi) for Targeted Gene Silencing in Mycobacteria
Methods in Molecular Biology, ISSN: 1940-6029, Vol: 2314, Page: 343-364
2021
- 21Citations
- 33Captures
- 1Mentions
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations21
- Citation Indexes21
- 21
- CrossRef7
- Captures33
- Readers33
- 33
- Mentions1
- News Mentions1
- News1
Book Chapter Description
The genetic basis for Mycobacterium tuberculosis pathogenesis is incompletely understood. One reason for this knowledge gap is the relative difficulty of genetic manipulation of M. tuberculosis. To close this gap, we recently developed a robust CRISPR interference (CRISPRi) platform for programmable gene silencing in mycobacteria. In this chapter, we: (1) discuss some of the advantages and disadvantages of CRISPRi relative to more traditional genetic approaches; and (2) provide a protocol for the application of CRISPRi to reduce transcription of target genes in mycobacteria.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85109927545&origin=inward; http://dx.doi.org/10.1007/978-1-0716-1460-0_16; http://www.ncbi.nlm.nih.gov/pubmed/34235662; https://link.springer.com/10.1007/978-1-0716-1460-0_16; https://dx.doi.org/10.1007/978-1-0716-1460-0_16; https://link.springer.com/protocol/10.1007/978-1-0716-1460-0_16
Springer Science and Business Media LLC
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