Identification of Essential Genes Using Sequential CRISPR and siRNA Screens
Methods in Molecular Biology, ISSN: 1940-6029, Vol: 2377, Page: 89-107
2022
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Book Chapter Description
Genome-wide CRISPR and siRNA screening methodologies are powerful tools that are aptly suited to the discovery of essential genes. In this chapter, we outline our methods to conduct sequential CRISPR and siRNA screens to quickly and efficiently identify essential genes within a collection of cell lines. The utilization of both screening methodologies provides a pipeline that minimizes costs and time while enabling the robust detection of candidate genes.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85118652658&origin=inward; http://dx.doi.org/10.1007/978-1-0716-1720-5_5; http://www.ncbi.nlm.nih.gov/pubmed/34709612; https://link.springer.com/10.1007/978-1-0716-1720-5_5; https://dx.doi.org/10.1007/978-1-0716-1720-5_5; https://link.springer.com/protocol/10.1007/978-1-0716-1720-5_5
Springer Science and Business Media LLC
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