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AIRR Community Guide to Planning and Performing AIRR-Seq Experiments

Methods in Molecular Biology, ISSN: 1940-6029, Vol: 2453, Page: 261-278
2022
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Book Chapter Description

The development of high-throughput sequencing of adaptive immune receptor repertoires (AIRR-seq of IG and TR rearrangements) has provided a new frontier for in-depth analysis of the immune system. The last decade has witnessed an explosion in protocols, experimental methodologies, and computational tools. In this chapter, we discuss the major considerations in planning a successful AIRR-seq experiment together with basic strategies for controlling and evaluating the outcome of the experiment. Members of the AIRR Community have authored several chapters in this edition, which cover step-by-step instructions to successfully conduct, analyze, and share an AIRR-seq project.

Bibliographic Details

Eugster, Anne; Bostick, Magnolia L; Gupta, Nidhi; Mariotti-Ferrandiz, Encarnita; Kraus, Gloria; Meng, Wenzhao; Soto, Cinque; Trück, Johannes; Stervbo, Ulrik; Luning Prak, Eline T; AIRR Community

Springer Science and Business Media LLC

Biochemistry, Genetics and Molecular Biology

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