FORK-seq: Single-Molecule Profiling of DNA Replication
Methods in Molecular Biology, ISSN: 1940-6029, Vol: 2477, Page: 107-128
2022
- 1Citations
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Book Chapter Description
Most genome replication mapping methods profile cell populations, masking cell-to-cell heterogeneity. Here, we describe FORK-seq, a nanopore sequencing method to map replication of single DNA molecules at 200 nucleotide resolution using a nanopore current interpretation tool allowing the quantification of BrdU incorporation. Along pulse-chased replication intermediates from Saccharomyces cerevisiae, we can orient replication tracks and reproduce population-based replication directionality profiles. Additionally, we can map individual initiation and termination events. Thus, FORK-seq reveals the full extent of cell-to-cell heterogeneity in DNA replication.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85129999687&origin=inward; http://dx.doi.org/10.1007/978-1-0716-2257-5_8; http://www.ncbi.nlm.nih.gov/pubmed/35524115; https://link.springer.com/10.1007/978-1-0716-2257-5_8; https://dx.doi.org/10.1007/978-1-0716-2257-5_8; https://link.springer.com/protocol/10.1007/978-1-0716-2257-5_8
Springer Science and Business Media LLC
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