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Autofluorescence Imaging to Evaluate Retinal Disease Progression in Rodent

Methods in Molecular Biology, ISSN: 1940-6029, Vol: 2560, Page: 229-232
2023
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Book Chapter Description

Fundus autofluorescence (FAF) imaging is a noninvasive retinal imaging methodology that allows mapping of lipofuscin distribution in the retinal pigment epithelium cell (RPE). Excessive accumulation of lipofuscin granules in the lysosomal compartment of RPE cells represents a common downstream pathogenetic pathway in various hereditary and complex retinal diseases, including age-related macular degeneration. The clinical applications of FAF coupled with its ease of use, and the noninvasive nature of characterizing retinal diseases, are increasingly valuable to the field of ophthalmology and in assessing the progression of retinitis pigmentosa (RP). Quantitative AF (qAF) enhances the understanding of retinal disease processes, serves as a diagnostic aid, and allows for the monitoring of the effects of therapeutic interventions. This chapter introduces basic principles of FAF and general protocols of FAF evaluating retinal disease progression in rodents.

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