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Chemical Synthesis of Non-hydrolyzable Ubiquitin(-Like) Hybrid Chains

Methods in Molecular Biology, ISSN: 1940-6029, Vol: 2602, Page: 41-49
2023
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Book Chapter Description

Hybrid chains are a combination of ubiquitin (Ub) and Ub-like (UbL) proteins, expanding on the finely tuned Ub code. To decipher this intricate code, understanding of its assembly, architecture, as well as specific interactors of these Ub/UbL hybrid chains are important, warranting the development of suitable reagents. Here, we describe the chemical methodology to access linkage specific non-hydrolyzable Ub-NEDD8-based chains endowed with an affinity handle in all possible combinations of K48 hybrid chain dimers between Ub and NEDD8.

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