Invadopodia Methods: Detection of Invadopodia Formation and Activity in Cancer Cells Using Reconstituted 2D and 3D Collagen-Based Matrices
Methods in Molecular Biology, ISSN: 1940-6029, Vol: 2608, Page: 225-246
2023
- 4Citations
- 4Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations4
- Citation Indexes4
- CrossRef2
- Captures4
- Readers4
Book Chapter Description
Tumor dissemination involves cancer cell migration through the extracellular matrix (ECM). ECM is mainly composed of collagen fibers that oppose cell invasion. To overcome hindrance in the matrix, cancer cells deploy a protease-dependent program in order to remodel the matrix fibers. Matrix remodeling requires the formation of actin-based matrix/plasma membrane contact sites called invadopodia, responsible for collagen cleavage through the accumulation and activity of the transmembrane type-I matrix metalloproteinase (MT1-MMP). In this article, we describe experimental procedures designed to assay for invadopodia formation and for invadopodia activity using 2D and 3D models based on gelatin (denatured collagen) and fibrillar type-I collagen matrices.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85146485694&origin=inward; http://dx.doi.org/10.1007/978-1-0716-2887-4_14; http://www.ncbi.nlm.nih.gov/pubmed/36653711; https://link.springer.com/10.1007/978-1-0716-2887-4_14; https://dx.doi.org/10.1007/978-1-0716-2887-4_14; https://link.springer.com/protocol/10.1007/978-1-0716-2887-4_14
Springer Science and Business Media LLC
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