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Assembly of the Mitochondrial Translation Initiation Complex

Methods in Molecular Biology, ISSN: 1940-6029, Vol: 2661, Page: 217-232
2023
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Book Chapter Description

Mitochondria maintain their own translational machinery that is responsible for the synthesis of essential components of the oxidative phosphorylation system. The mammalian mitochondrial translation system differs significantly from its cytosolic and bacterial counterparts. Here, we describe detailed protocols for efficient in vitro reconstitution of the mammalian mitochondrial translation initiation complex, which can be further used for mechanistic analyses of different aspects of mitochondrial translation.

Bibliographic Details

Remes, Cristina; Nguyen, Minh Duc; Spahr, Henrik; Ng, Martin; Fritsch, Clark; Bhattacharya, Arpan; Li, Hong; Cooperman, Barry; Rorbach, Joanna

Springer Science and Business Media LLC

Biochemistry, Genetics and Molecular Biology

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