FACS-Based Sequencing Approach to Evaluate Cell Type to Genotype Associations Using Cerebral Organoids
Methods in Molecular Biology, ISSN: 1940-6029, Vol: 2683, Page: 193-199
2023
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Book Chapter Description
Recent technological developments have led to widespread applications of large-scale transcriptomics-based sequencing methods to identify genotype-to-cell type associations. Here we describe a fluorescence-activated cell sorting (FACS)-based sequencing method to utilize CRISPR/Cas9 edited mosaic cerebral organoids to identify or validate genotype-to-cell type associations. Our approach is high-throughput and quantitative and uses internal controls to enable comparisons of the results across different antibody markers and experiments.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85163625432&origin=inward; http://dx.doi.org/10.1007/978-1-0716-3287-1_15; http://www.ncbi.nlm.nih.gov/pubmed/37300776; https://link.springer.com/10.1007/978-1-0716-3287-1_15; https://dx.doi.org/10.1007/978-1-0716-3287-1_15; https://link.springer.com/protocol/10.1007/978-1-0716-3287-1_15
Springer Science and Business Media LLC
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