A Streamlined Method for Detecting Inflammasome-Induced ASC Oligomerization Using Chemical Crosslinking
Methods in Molecular Biology, ISSN: 1940-6029, Vol: 2691, Page: 155-164
2023
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Book Chapter Description
The apoptosis-associated speck-like protein containing a caspase activation and recruitment domain (ASC) functions as the integral adaptor protein between inflammasome sensors such as NOD-like receptor protein 3 (NLRP3) and the inflammatory caspase, caspase-1. Inflammasome sensor triggering allows recruitment of ASC and the formation of long amyloid-like ASC oligomers that enable binding and proximity-induced activation of caspase-1. The detection of ASC oligomerization thus constitutes a highly specific and direct test for inflammasome complex formation and activation. Here, we describe a simplified and streamlined method for the detection of ASC oligomers via Western blotting, using the chemical crosslinking reagent disuccinimidyl suberate.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85162804687&origin=inward; http://dx.doi.org/10.1007/978-1-0716-3331-1_12; http://www.ncbi.nlm.nih.gov/pubmed/37355544; https://link.springer.com/10.1007/978-1-0716-3331-1_12; https://dx.doi.org/10.1007/978-1-0716-3331-1_12; https://link.springer.com/protocol/10.1007/978-1-0716-3331-1_12
Springer Science and Business Media LLC
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