CUT&Tag for Mapping In Vivo Protein-DNA Interactions in Plants
Methods in Molecular Biology, ISSN: 1940-6029, Vol: 2698, Page: 109-117
2023
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Book Chapter Description
Histone post-translational modifications and transcription factors (TFs) play vital roles in regulating gene expression. A comprehensive understanding of transcriptional regulation requires genome-wide mapping of chromatin features such as histone modifications and TF binding sites. Here, we describe a detailed nucleus CUT&Tag (Cleavage Under Targets and Tagmentation) protocol, which is an antibody-guided in situ protein-DNA interaction mapping method using protein A/G fused Tn5 transposase. Compared with regular ChIP-seq in plants, nucleus CUT&Tag (nCUT&Tag) omits many steps such as sonication and immunoprecipitation, thus saving much time and making it possible to efficiently profile chromatin features from low-input and even single cells with higher signal-to-noise ratio.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85170178860&origin=inward; http://dx.doi.org/10.1007/978-1-0716-3354-0_8; http://www.ncbi.nlm.nih.gov/pubmed/37682472; https://link.springer.com/10.1007/978-1-0716-3354-0_8; https://dx.doi.org/10.1007/978-1-0716-3354-0_8; https://link.springer.com/protocol/10.1007/978-1-0716-3354-0_8
Springer Science and Business Media LLC
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