Type I Lipoteichoic Acid (LTA) Detection by Western Blot
Methods in Molecular Biology, ISSN: 1940-6029, Vol: 2727, Page: 95-106
2024
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Book Chapter Description
Type I lipoteichoic acid (LTA) is a glycerol phosphate polymer found in the cell envelope of diverse Gram-positive bacteria including Staphylococcus aureus, Bacillus subtilis, and Listeria monocytogenes. The polymer is linked by a lipid anchor to the outer leaflet of the bacterial membrane and in some bacteria can also be shed and detected in the culture supernatant. Here, we describe a simple and rapid western blot method for the detection of Type I LTA in bacterial cell extracts and culture supernatant fractions using a polyglycerol phosphate specific monoclonal LTA antibody.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85174931674&origin=inward; http://dx.doi.org/10.1007/978-1-0716-3491-2_8; http://www.ncbi.nlm.nih.gov/pubmed/37815711; https://link.springer.com/10.1007/978-1-0716-3491-2_8; https://dx.doi.org/10.1007/978-1-0716-3491-2_8; https://link.springer.com/protocol/10.1007/978-1-0716-3491-2_8
Springer Science and Business Media LLC
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