Benefits and limitations of protein hydrolysates as components of serum-free media for animal cell culture applications protein hydrolysates in serum free media
Protein Hydrolysates in Biotechnology, Page: 55-78
2010
- 20Citations
- 60Captures
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Book Chapter Description
Increased understanding of influential factors for the cultivation of animal cells, combined with heightened regulatory concern over potential transmission of adventitious contaminants associated with serum and other animal-derived components, has elevated interest in using protein hydrolysates as serum replacements or nutrient supplements. This paper reviews the chemistry and biology of various hydrolysates derived from animal, plant and microbial sources. It provides specific examples of a beneficial selection of plant and yeast hydrolysates as ingredients of serum-free nutrient formulations for bioproduction applications of cultured mammalian and insect cells. Strategies for customizing and optimizing nutrients for specialized applications and general benefits and limitations of protein hydrolysates for biopharmaceutical production are also discussed. © 2010 Springer Science+Business Media B.V.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84892252023&origin=inward; http://dx.doi.org/10.1007/978-1-4020-6674-0_4; http://link.springer.com/10.1007/978-1-4020-6674-0_4; http://link.springer.com/content/pdf/10.1007/978-1-4020-6674-0_4.pdf; https://dx.doi.org/10.1007/978-1-4020-6674-0_4; https://link.springer.com/chapter/10.1007/978-1-4020-6674-0_4; http://www.springerlink.com/index/10.1007/978-1-4020-6674-0_4; http://www.springerlink.com/index/pdf/10.1007/978-1-4020-6674-0_4
Springer Science and Business Media LLC
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