Recombinant expression and purification of "virus-like" bacterial encapsulin protein cages
Methods in molecular biology (Clifton, N.J.), ISSN: 1940-6029, Vol: 1252, Page: 61-67
2015
- 9Citations
- 19Captures
Metric Options: CountsSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations9
- Citation Indexes9
- CrossRef9
- Captures19
- Readers19
- 19
Book Chapter Description
Ultracentrifugation, particularly the use of sucrose or cesium chloride density gradients, is a highly reliable and efficient technique for the purification of virus-like particles and protein cages. Since virus-like particles and protein cages have a unique size compared to cellular macromolecules and organelles, the rate of migration can be used as a tool for purification. Here we describe a detailed protocol for the purification of recently discovered virus-like assemblies called bacterial encapsulins from Thermotoga maritima and Brevibacterium linens.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84955162859&origin=inward; http://dx.doi.org/10.1007/978-1-4939-2131-7_6; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84922021348&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/25358773; https://link.springer.com/10.1007/978-1-4939-2131-7_6; https://dx.doi.org/10.1007/978-1-4939-2131-7_6; https://link.springer.com/protocol/10.1007/978-1-4939-2131-7_6
Springer Science and Business Media LLC
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