A yeast-based high-throughput screen for modulators of phosphodiesterase activity
cAMP Signaling: Methods and Protocols, ISSN: 1064-3745, Vol: 1294, Page: 181-190
2015
- 3Citations
- 3Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations3
- Citation Indexes3
- CrossRef3
- Captures3
- Readers3
Book Chapter Description
Cell-based high-throughput screens (HTSs) targeting heterologously expressed proteins in yeast identify compounds that often display relevant biological activity when tested in cell culture. We developed a fission yeast-based HTS to detect small-molecule inhibitors of mammalian cyclic nucleotide phosphodiesterases (PDEs). These screens are carried out in Schizosaccharomyces pombe using a PKA-repressed fbp1-ura4 reporter whose expression due to low PKA activity prevents cells from growing in medium containing the pyrimidine analog 5-fluoro orotic acid (5FOA). We describe here the steps required to construct strains for screening and to optimize conditions for successful screens.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84958651353&origin=inward; http://dx.doi.org/10.1007/978-1-4939-2537-7_14; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84925307558&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/25783886; https://link.springer.com/10.1007/978-1-4939-2537-7_14; https://dx.doi.org/10.1007/978-1-4939-2537-7_14; https://link.springer.com/protocol/10.1007/978-1-4939-2537-7_14
Springer Science and Business Media LLC
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