Global analysis of DNA 5-methylcytosine using the luminometric methylation assay, LUMA
Methods in Molecular Biology, ISSN: 1064-3745, Vol: 1315, Page: 209-219
2015
- 7Citations
- 8Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations7
- Citation Indexes7
- CrossRef6
- Captures8
- Readers8
Book Chapter Description
The study of epigenetic alterations of the genome is becoming increasingly important in order to understand how environment and genetic background interact to build and regulate the functional genome. There are several types of epigenetic modifications to both DNA and histone proteins in eukaryotic cells; chiefly studied among these are changes to cytosine, where methylation of the 5-carbon position is the most prominent. Although this has many consequences for gene regulation and cell differentiation, other modifications have recently emerged as biologically relevant. Since global DNA methylation states may be used as a general measure of the methylome, cost-effective, rapid, and specific analytical tools are wanted. This protocol described here focuses on the Luminometric Methylation Assay (LUMA), a method which analyzes global DNA 5-methylcytosine (5mC) through the use of restriction enzymes and detection with Pyrosequencing ® . Up to 96 samples can be simultaneously analyzed. In contrast to the majority of other methods focused on 5mC analysis, with appropriate enzymes, LUMA does not appear to detect 5-hydroxymethylcytosine (5hmC) and is therefore more specific than most 5mC techniques.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84933060148&origin=inward; http://dx.doi.org/10.1007/978-1-4939-2715-9_16; http://www.ncbi.nlm.nih.gov/pubmed/26103902; https://link.springer.com/10.1007/978-1-4939-2715-9_16; https://dx.doi.org/10.1007/978-1-4939-2715-9_16; https://link.springer.com/protocol/10.1007/978-1-4939-2715-9_16
Springer Science and Business Media LLC
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