Simultaneous quantifi cation of global 5mc and 5hmc levels in the nervous system using an hplc/ms method
Neuromethods, ISSN: 1940-6045, Vol: 105, Page: 87-96
2016
- 2Captures
Metric Options: CountsSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Captures2
- Readers2
Book Chapter Description
Methylation of DNA at the 5′ carbon of cytosine (5mC) is a key epigenetic regulator of gene expression required for the proper development and function of the central nervous system. Observations of experience-dependent fl uctuations in the patterns of 5mC at neuronal gene enhancers, promoters and intragenic regions have prompted great interest in the role of DNA methylation mechanisms in the brain. In addition, the recent discovery of 5-hydroxymethylcytosine (5hmC), an oxidized derivative of 5mC that acts as a DNA demethylation intermediate has offered mechanistic insight into how these changes in 5mC levels are achieved. As a result, numerous techniques have now been adapted or newly developed to quantify and profi le 5mC and 5hmC in the genome. Here we describe a method using high performance liquid chromatography electrospray ionization tandem mass spectrometry with multiple reaction monitoring (HPLC-ESI-MS/MS-MRM) for the simultaneous detection and quantifi cation of global 5mC and 5hmC levels present in genomic DNA from nervous-system derived samples. HPLC-ESI-MS/MS-MRM analysis offers the highest levels of sensitivity, selectivity, and precision available for the determination of global changes in these epigenetic modifi cations.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84945219221&origin=inward; http://dx.doi.org/10.1007/978-1-4939-2754-8_5; https://link.springer.com/10.1007/978-1-4939-2754-8_5; https://dx.doi.org/10.1007/978-1-4939-2754-8_5; https://link.springer.com/protocol/10.1007/978-1-4939-2754-8_5
Springer Science and Business Media LLC
Provide Feedback
Have ideas for a new metric? Would you like to see something else here?Let us know