Regulation of heart contractility by M and M muscarinic receptors: Functional studies using muscarinic receptor knockout mouse
Neuromethods, ISSN: 1940-6045, Vol: 107, Page: 235-259
2016
- 3Citations
- 5Captures
Metric Options: CountsSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Book Chapter Description
To investigate the functional roles of M and M muscarinic receptors in mouse atria, wild-type mice, muscarinic M or M single receptor knockout mice (M KO, M KO), and M and M muscarinic receptor double knockout mice (M /M KO) were used for pharmacological and molecular biological approaches. Effects of carbachol on spontaneous contraction (right atrium) or electrically evoked contraction (left atrium) were examined in the isolated atria of the respective mice. Presence of muscarinic receptor subtype mRNAs and proteins was determined by real time RT-PCR using specifi c primers and immunohistochemistry using specifi c anti-M and anti-M receptor antibodies. Quantitative real-time RT-PCR analysis showed that M 2 receptor mRNA was expressed dominantly in mouse atria but that the M, M, M, and M receptor subtypes were also expressed at low levels. Carbachol decreased the frequency of spontaneous beating in right atria of mice through activation of the M receptor subtype. In left atria of wild-type mice, carbachol decreased the amplitude of electrical field stimulation (EFS)-evoked contractions (M receptors), but this inhibition was transient and was followed by a gradual increase in contraction amplitude (M receptors). Cyclooxygenase-2 (COX-2) and prostaglandins in the endocardial endothelium were involved in the M receptor-mediated positive inotropic actions. In conclusion, the present studies using isolated atria of muscarinic receptor knockout mice demonstrated that myocardial M receptors mediate negative chronotropic/inotropic actions and that M muscarinic receptors mediate positive chronotropic/inotropic actions in mouse atria. Physiologically, M receptor-mediated excitatory cardiac effects might dampen the inhibitory effects of M receptor activation on cardiac contractility.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84938704876&origin=inward; http://dx.doi.org/10.1007/978-1-4939-2858-3_13; https://link.springer.com/10.1007/978-1-4939-2858-3_13; https://dx.doi.org/10.1007/978-1-4939-2858-3_13; https://link.springer.com/protocol/10.1007/978-1-4939-2858-3_13
Springer Science and Business Media LLC
Provide Feedback
Have ideas for a new metric? Would you like to see something else here?Let us know