Preparation and fluorescent analysis of plant metaphase chromosomes
Methods in Molecular Biology, ISSN: 1064-3745, Vol: 1370, Page: 87-103
2015
- 17Citations
- 38Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations17
- Citation Indexes17
- 17
- CrossRef8
- Captures38
- Readers38
- 38
Book Chapter Description
Good preparations are essential for informative analysis of both somatic and meiotic chromosomes, cytogenetics, and cell divisions. Fluorescent chromosome staining allows even small chromosomes to be visualized and counted, showing their morphology. Aneuploidies and polyploidies can be established for species, populations, or individuals while changes occurring in breeding lines during hybridization or tissue culture and transformation protocols can be assessed. The process of division can be followed during mitosis and meiosis including pairing and chiasma distribution, as well as DNA organization and structure during the evolution of chromosomes can be studied. This chapter presents protocols for pretreatment and fixation of material, including tips of how to grow plants to get good and healthy meristem with many divisions. The chromosome preparation technique is described using proteolytic enzymes, but acids can be used instead. Chromosome slide preparations are suitable for fluorochrome staining for fast screening described in the chapter or fluorescent in situ hybridization (see Schwarzacher and Heslop-Harrison, In situ hybridization. BIOS Scientifi c Publishers, Oxford, 2000
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84949774218&origin=inward; http://dx.doi.org/10.1007/978-1-4939-3142-2_7; http://www.ncbi.nlm.nih.gov/pubmed/26659956; https://link.springer.com/10.1007/978-1-4939-3142-2_7; https://doi.org/10.1007%2F978-1-4939-3142-2_7; https://dx.doi.org/10.1007/978-1-4939-3142-2_7; https://link.springer.com/protocol/10.1007/978-1-4939-3142-2_7
Springer Science and Business Media LLC
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