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Detection of isolated mitochondria-associated ER membranes using the sigma-1 receptor

Methods in Molecular Biology, ISSN: 1064-3745, Vol: 1376, Page: 133-140
2016
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Book Chapter Description

The interface between the endoplasmic reticulum (ER) and mitochondria referred to as the MAM (mitochondria- associated ER m embrane) plays important roles in many physiological functions. A specific marker for this important entity of cellular structure is urgently needed. Thus, we propose in this method chapter that the membrane-bound ER chaperone sigma-1 receptor serves as an ideal marker for the MAM. We describe in detail the preparation and purification of the MAM by using the sigma-1 receptor as the marker and demonstrate the uniqueness of this marker by using a variety of cells, peripheral and neuronal.

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