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Screening for drugs against the plasmodium falciparum digestive vacuole by imaging flow cytometry

Methods in Molecular Biology, ISSN: 1064-3745, Vol: 1389, Page: 195-2015
2016
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Book Chapter Description

Phenotypic assays are increasingly employed to provide clues about drug mechanisms. In antimalarial drug screening, however, the majority of assays are designed to only measure parasite-killing activity. We describe here a high-content assay to detect drug-mediated perturbation of the digestive vacuole integrity in the trophozoite stage of Plasmodium falciparum using the ImageStream imaging flow cytometer.

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