Quantitative evaluation of plant actin cytoskeletal organization during immune signaling
Methods in Molecular Biology, ISSN: 1064-3745, Vol: 1578, Page: 207-221
2017
- 9Citations
- 8Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations9
- Citation Indexes9
- CrossRef2
- Captures8
- Readers8
Book Chapter Description
High spatial and temporal resolution microscopy-based methods are valuable tools for the precise real-time imaging of changes in cellular organization in response to stimulus perception. Here, we describe a quantitative method for the evaluation of the plant actin cytoskeleton during immune stimulus perception and the activation of defense signaling. As a measure of the biotic stress-induced changes in actin filament organization, we present methods for analyzing changes in actin filament organization following elicitation of pattern-triggered immunity and effector-triggered immunity. Using these methods, it is possible to not only quantitatively evaluate changes in actin cytoskeletal organization following biotic stress perception, but to also use these protocols to assess changes in actin filament organization following perception of a wide range of stimuli, including abiotic and developmental cues. As described herein, we present an example application of this method, designed to evaluate changes in actin cytoskeletal organization following pathogen perception and immune signaling.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85013633896&origin=inward; http://dx.doi.org/10.1007/978-1-4939-6859-6_17; http://www.ncbi.nlm.nih.gov/pubmed/28220427; http://link.springer.com/10.1007/978-1-4939-6859-6_17; https://doi.org/10.1007%2F978-1-4939-6859-6_17; https://dx.doi.org/10.1007/978-1-4939-6859-6_17; https://link.springer.com/protocol/10.1007/978-1-4939-6859-6_17
Springer Science and Business Media LLC
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