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Cold-shock expression system in E. coli for protein NMR studies

Methods in Molecular Biology, ISSN: 1064-3745, Vol: 1586, Page: 345-357
2017
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Book Chapter Description

The cold-shock system using the pCold vector is one of the most effective Escherichia coli heterologous protein expression systems. It allows the improvement of the expression level of the protein of interest in a soluble fraction. In this chapter, we describe practical procedures for the overexpression of heterologous protein of interest by using the pCold vector or the single-protein production system. The latter is one of the most advanced pCold technologies for isotope labeling of the target protein and its NMR studies.

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