Cold-shock expression system in E. coli for protein NMR studies
Methods in Molecular Biology, ISSN: 1064-3745, Vol: 1586, Page: 345-357
2017
- 4Citations
- 10Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations4
- Citation Indexes4
- Captures10
- Readers10
- 10
Book Chapter Description
The cold-shock system using the pCold vector is one of the most effective Escherichia coli heterologous protein expression systems. It allows the improvement of the expression level of the protein of interest in a soluble fraction. In this chapter, we describe practical procedures for the overexpression of heterologous protein of interest by using the pCold vector or the single-protein production system. The latter is one of the most advanced pCold technologies for isotope labeling of the target protein and its NMR studies.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85018448632&origin=inward; http://dx.doi.org/10.1007/978-1-4939-6887-9_23; http://www.ncbi.nlm.nih.gov/pubmed/28470617; http://link.springer.com/10.1007/978-1-4939-6887-9_23; https://dx.doi.org/10.1007/978-1-4939-6887-9_23; https://link.springer.com/protocol/10.1007/978-1-4939-6887-9_23
Springer Science and Business Media LLC
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