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Microdissection of Alzheimer brain tissue for the determination of focal manganese accumulation

Neuromethods, ISSN: 1940-6045, Vol: 124, Page: 109-118
2017
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    Citations
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  • 8
    Captures
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  • 37
    Social Media
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  • Citations
    1
  • Captures
    8
  • Social Media
    37
    • Shares, Likes & Comments
      37
      • Facebook
        37

Book Chapter Description

This protocol utilizes immunohistochemistry to assist the laser microdissection and capture of tissue regions of interest isolated from slide-mounted sections for subsequent analysis of metal content. When used in conjunction with inductively coupled plasma mass spectrometry it becomes a powerful method to determine pathogenetically related shifts in the concentration of various cortical transition metals. Key advantages of this approach include being able to isolate target and directly adjacent brain regions for easy comparative analysis free from potential metal contamination as can occur during surgically based isolation of tissue elements. In this chapter, I present a well-optimized and validated method to achieve contamination-free detection of microscopically defined regions of manganese concentration in Alzheimer’s disease brain tissue with an emphasis on amyloid plaque analysis.

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