PlumX Metrics
Embed PlumX Metrics

Small RNA sequencing: A technique for miRNA profiling

Methods in Molecular Biology, ISSN: 1064-3745, Vol: 1735, Page: 321-330
2018
  • 9
    Citations
  • 0
    Usage
  • 30
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

Book Chapter Description

Identifying microRNA (miRNA) signatures in animal tissues is an essential first step in studies assessing post-transcriptional regulation of gene expression in health or disease. Small RNA sequencing (sRNA-Seq) is a next-generation sequencing-based technology that is currently considered the most powerful and versatile tool for miRNA profiling. Here, we describe a sRNA-Seq protocol including RNA purification from mammalian tissues, library preparation, and raw data analysis.

Provide Feedback

Have ideas for a new metric? Would you like to see something else here?Let us know