Clonal analysis of planarian stem cells by subtotal irradiation and single-cell transplantation
Methods in Molecular Biology, ISSN: 1064-3745, Vol: 1774, Page: 479-495
2018
- 8Citations
- 18Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations8
- Citation Indexes8
- CrossRef6
- Captures18
- Readers18
- 18
Book Chapter Description
Stem cells, which both self-renew and produce differentiated progeny, represent fundamental biological units for the development, growth, maintenance, and regeneration of adult tissues. Characterization of stem cell lineage potential can be accomplished with clonal assays that interrogate stem cell output at the single-cell level. Here we present two methods for clonal analysis of individual proliferative cells (neoblasts) in the planarian Schmidtea mediterranea. The first method utilizes “subtotal” gamma irradiation to study rare surviving neoblasts and their clonal descendants in their native environment. The second method utilizes a fluorescent-activated cell sorting (FACS) strategy to obtain neoblast-enriched cell fractions, followed by single-cell transplantation into lethally irradiated hosts. Together, these methods provide a framework for generation and analysis of stem cell-derived clones in planarians.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85048855733&origin=inward; http://dx.doi.org/10.1007/978-1-4939-7802-1_20; http://www.ncbi.nlm.nih.gov/pubmed/29916173; http://link.springer.com/10.1007/978-1-4939-7802-1_20; https://dx.doi.org/10.1007/978-1-4939-7802-1_20; https://link.springer.com/protocol/10.1007/978-1-4939-7802-1_20
Springer Science and Business Media LLC
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