Production of proteins for NMR studies using the wheat germ cell-free system.
Methods in molecular biology (Clifton, N.J.), ISSN: 1064-3745, Vol: 310, Page: 169-185
2005
- 10Citations
- 10Captures
Metric Options: CountsSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations10
- Citation Indexes10
- 10
- CrossRef7
- Captures10
- Readers10
- 10
Book Chapter Description
This chapter describes protocols for preparing 15N-labeled proteins (ubiquitin is used as an example) using Escherichia coli cells (with purification) and the wheat germ cell-free system (without purification). A comparison of 1H-15N heteronuclear single-quantum coherence (HSQC) spectra of yeast ubiquitin prepared using each method indicates that this wheat germ cell-free system may be used for rapid nuclear magnetic resonance analyses of proteins without purification.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=33644793335&origin=inward; http://dx.doi.org/10.1007/978-1-59259-948-6_12; http://www.ncbi.nlm.nih.gov/pubmed/16350954; http://link.springer.com/10.1007/978-1-59259-948-6_12; http://www.springerlink.com/index/10.1007/978-1-59259-948-6_12; http://www.springerlink.com/index/pdf/10.1007/978-1-59259-948-6_12; https://dx.doi.org/10.1007/978-1-59259-948-6_12; https://link.springer.com/protocol/10.1007/978-1-59259-948-6_12
Springer Science and Business Media LLC
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