SNP detection using trityl mass tags.
Methods in molecular biology (Clifton, N.J.), ISSN: 1940-6029, Vol: 578, Page: 345-361
2009
- 3Citations
- 36Captures
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Book Chapter Description
A new method suitable for single nucleotide polymorphism (SNP) detection using differential oligonucleotide probe extension has been developed. Sulfur-linked laser-cleavable trityl labels are implemented in this protocol. The method is based on mass spectrometry and utilizes a single surface for affinity purification of extended probes and matrix-independent desorption-ionization of the cleavable labels. The usefulness of this method for SNP genotyping is demonstrated.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=73449090172&origin=inward; http://dx.doi.org/10.1007/978-1-60327-411-1_21; http://www.ncbi.nlm.nih.gov/pubmed/19768604; http://link.springer.com/10.1007/978-1-60327-411-1_21; http://www.springerlink.com/index/10.1007/978-1-60327-411-1_21; http://www.springerlink.com/index/pdf/10.1007/978-1-60327-411-1_21; https://dx.doi.org/10.1007/978-1-60327-411-1_21; https://link.springer.com/protocol/10.1007/978-1-60327-411-1_21
Springer Science and Business Media LLC
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