Preparation of pichia pastoris expression plasmids
Methods in Molecular Biology, ISSN: 1064-3745, Vol: 866, Page: 25-40
2012
- 11Citations
- 52Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations11
- Citation Indexes11
- 11
- CrossRef8
- Captures52
- Readers52
- 52
Book Chapter Description
When planning any heterologous expression experiment, the very first critical step is related to the design of the overall strategy, hence to the selection of the most adapted expression vector. The very flexible Pichia pastoris system offers a broad range of possibilities for the production of secreted, endogenous or membrane proteins thanks to a combination of various plasmid backbones, selection markers, promoters and fusion sequences introduced into dedicated host strains. The present chapter provides some guidelines on the choice of expression vectors and expression strategies. It also brings the reader a complete toolbox from which plasmids and fusion sequences can be picked and assembled to set up appropriate expression vectors. Finally, it provides standard starting protocols for the preparation of the selected plasmids and their use for host strain transformation. © 2012 Springer Science+business Media, LLC.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84859809920&origin=inward; http://dx.doi.org/10.1007/978-1-61779-770-5_3; http://www.ncbi.nlm.nih.gov/pubmed/22454111; http://link.springer.com/10.1007/978-1-61779-770-5_3; https://doi.org/10.1007%2F978-1-61779-770-5_3; https://dx.doi.org/10.1007/978-1-61779-770-5_3; https://link.springer.com/protocol/10.1007/978-1-61779-770-5_3; http://www.springerlink.com/index/10.1007/978-1-61779-770-5_3; http://www.springerlink.com/index/pdf/10.1007/978-1-61779-770-5_3
Springer Science and Business Media LLC
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