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Site-specific labeling of his-tagged nanobodies with Tc: A practical guide

Methods in Molecular Biology, ISSN: 1064-3745, Vol: 911, Page: 485-490
2012
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Book Chapter Description

99mTc-tricarbonyl chemistry provides an elegant technology to site-specifically radiolabel histidine-tagged biomolecules. Considering their unique biochemical properties, this straightforward technology is particularly suited for Nanobodies. This chapter gives a detailed guide to generate highly specific Nanobody-derived radiotracers for both in vitro binding studies and in vivo molecular imaging. © 2012 Springer Science+Business Media, LLC.

Bibliographic Details

Xavier, Catarina; Devoogdt, Nick; Hernot, Sophie; Vaneycken, Ilse; D'Huyvetter, Matthias; De Vos, Jens; Massa, Sam; Lahoutte, Tony; Caveliers, Vicky

Springer Science and Business Media LLC

Biochemistry, Genetics and Molecular Biology

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