Biochemical analysis of the interaction between phospholipase Dα1 and GTP-binding protein α-subunit from Arabidopsis thaliana
Methods in Molecular Biology, ISSN: 1064-3745, Vol: 1043, Page: 21-35
2013
- 11Citations
- 8Captures
Metric Options: CountsSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations11
- Citation Indexes11
- 11
- CrossRef9
- Captures8
- Readers8
Book Chapter Description
Phospholipase Ds (PLDs) play diverse roles in plant lipid metabolism and cellular signaling processes. The sole canonical G-protein α-subunit (Gα) in Arabidopsis also plays multiple roles in plant growth and cellular signaling processes. Interestingly, overlapping functions of PLD and Gα have been indicated in many cellular processes, including abscisic acid (ABA)-mediated stomata movement and water loss, gibberellic acid (GA)-regulated seed germination, and auxin signaling. This interaction between PLD and Gα has also been suggested in biochemical and physiological studies. Here we described the methods used for studying the interaction between the major PLD form PLDα1 and Gα. From pulldown experiments with purified bacterially expressed PLDα1 and Gα, co-immunoprecipitation of plant protein extract, and yeast two-hybrid assay, we showed that there is a physical interaction between PLDα1 and Gα, and identified a key DRY motif in PLDα1, which is an essential element for the interaction. The interaction of PLDα1 and Gα can be affected by factors like GTP or GDP, but it also affected PLD phospholipase activity and Gα GTPase activity in turn. © Springer Science+Business Media, LLC 2013.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84922827769&origin=inward; http://dx.doi.org/10.1007/978-1-62703-532-3_3; http://www.ncbi.nlm.nih.gov/pubmed/23913032; https://link.springer.com/10.1007/978-1-62703-532-3_3; https://dx.doi.org/10.1007/978-1-62703-532-3_3; https://link.springer.com/protocol/10.1007/978-1-62703-532-3_3
Springer Science and Business Media LLC
Provide Feedback
Have ideas for a new metric? Would you like to see something else here?Let us know