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VE-cadherin status as an indicator of microvascular permeability

Methods in Molecular Biology, ISSN: 1064-3745, Vol: 1046, Page: 335-342
2013
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Book Chapter Description

VE-cadherin phosphorylation and binding partner status are important indicators of endothelial permeability. Here we describe several techniques aimed at discerning total tyrosine phosphorylation levels of VE-cadherin, VE-cadherin phosphorylation on specific tyrosine residues, and the ability of VE-cadherin to bind its binding partner beta-catenin. Taken together, these approaches to studying VE-cadherin status on microvascular endothelial cells are excellent complements to traditional permeability assays. © Springer Science+Business Media, LLC 2013.

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