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In Vitro Perfusion of the Whole Ovary

Female and Male Fertility Preservation, Page: 369-373
2022
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Book Chapter Description

The creation of an in vitro whole ovary perfusion system allows to study morphology, structure, and function of tissue in fine detail since cell communications are kept intact. To establish the ovarian in vitro perfusion system, the whole ovary is inserted into a bioreactor where the organ is connected to a peristaltic perfusion apparatus and kept for a variable number of hours/days. Hormone production and ovulation have been studied using animal experimental models. Mature rabbit oocytes have been retrieved during the in vitro perfusion and then fertilized in vitro, which resulted in offspring. Furthermore, metaphase I sheep oocytes have been obtained, and ovarian hormonal production has been documented. The next step is to utilize in vitro perfusion of whole human ovaries to induce in vitro follicle growth and obtain mature metaphase II oocytes that can be cryopreserved or fertilized as a means of fertility preservation when ovarian stimulation in vivo is not feasible.

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