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Escherichia Coli LEGM-18 and ŽP Strains: Biological Properties Associated with Probiotic Potential

Lecture Notes in Networks and Systems, ISSN: 2367-3389, Vol: 622 LNNS, Page: 556-565
2023
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Conference Paper Description

Strains of various bacterial species and their products, which are involved in the decomposition of complex substances, are used for the formation and maintenance of a balanced host’s gut microbiota. The biological properties (bacterial cell surface hydrophobicity, adhesion, antagonistic activity towards other strains and resistance to biological fluids) of two Escherichia coli strains, LEGM-18 (a natural strain, isolated from a healthy person) and ŽP (a genetically modified Nissle 1917 strain that harbors a conjugative plasmid with the ColE7 bacteriocin gene) were studied. The cell surface hydrophobicity of both investigated strains was relatively low. In the adhesion assays, the LEGM-18 strain exhibited a moderate adhesion to human erythrocytes, while the ŽP exhibited low adhesion. Both strains had significant antagonistic effect against E. coli O157, E. coli 643 and Shigella flexneri. LEGM-18, as well as ŽP, were resistant to pepsin and bile action. The data obtained indicate that LEGM-18 and ŽP have potential as new veterinary probiotics.

Bibliographic Details

Marina V. Kuznetsova; Larisa Yu. Nesterova; Irina L. Maslennikova; Elizaveta V. Afanasevskaya; Valeriy A. Neschislyaev; Marjanca Starčič Erjavec

Springer Science and Business Media LLC

Engineering; Computer Science

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