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Rebinding of Proximal Histidine in the Cytochrome c' from Alcaligenes xylosoxidans Acts as a Molecular Trap for Nitric Oxide

Springer Series in Chemical Physics, ISSN: 0172-6218, Vol: 92, Page: 556-558
2009
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Book Chapter Description

Transient absorption spectra on cytochrome c’ and their kinetics were recorded to identify the formation of 5-coordinate (5c)-NO and 5c-His hemes from 4c-heme (99% and 1% amplitudes; 7-ps and 100-ps time constants, respectively). We demonstrate that proximal histidine precludes NO rebinding at the proximal site.

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